Protein_Domain

Part:BBa_K909007:Design

Designed by: Gintautas Vainorius   Group: iGEM12_ETH_Zurich   (2012-09-23)

TetR DNA binding domain containing BamHI site for protein fusions


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 382
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Deletion of alpha 8-10 helices might lower the stability of protein, also TetR-DBD is no longer responsive to anhydrotetracycline

Source

E. coli Tn10 transposon

References

Moncivais K, Zhang ZJ., Tetracycline repressor-based mammalian two-hybrid systems. Methods in Molecular Biology, 2012, Volume 812, 259-273.